So you want to do a: PacBio RNAseq Experiment

Basic Information:

Friday Sept 27, 2024 @ 11:00 - 12:00 

Organizer - UCSF CAT Bioinformatics, Dr. Matt Settles Managing Director, Bioinformatics

Contact - [email protected]

Location - Genentech North 114

Cost - FREE

Registration: So you want to do a: PacBio RNASEQ experiment 

Following the Seminar representative from PacBio will be available for "PacBio Coffee Hour" 

Seminar Description:

This FREE workshop is intended for those who are interested in and/or are in the planning stages of conducting a long-read RNA-Seq (bulk or single cell) experiment.  Topics to be discussed will include:

  • Experimental Design of RNA-Seq experiment
  • Sample preparation, best practices
  • Long-read sequencing vs. short-read sequencing
  • Discussion of PacBio Kinnex and the advantages of
  • Cost estimation
  • Raw data processing to count tables (the basic of)
  • Differential Gene Expression Analysis
  • Downstream analysis/visualizations and tables
  • What do you gain? long-reads vs short-reads

Referencing the CAT Core and Publications

 

When submitting manuscripts, please acknowledge the CAT by including the text: “Sequencing was performed at the UCSF CAT, supported by UCSF PBBR, RRP IMIA, and NIH 1S10OD028511-01 grants.” 

 

To aid our facility in future grant submissions, it helps to reference labs and publications that have benefited from our core please notify us of your achievement by emailing catcore [at] ucsf.edu the PMID number, lab, and the piece of equipment used in the paper.