Library Preparation

We currently offer the following sequencing full and library preparation services.

If you need bioinformatics support, please consult with our bioinformatics team before starting your project to ensure optimal study design and analysis compatibility.

Current Full and Library Preparation Services

Full-Service Illumina 30x Human Whole Genome Sequencing (WGS) 

- Cost: $456.25  (extraction, library prep and sequencing)
- Details: This service includes DNA extraction, Illumina library preparation, and sequencing on NovaSeq 25B flow cells. Samples must be submitted in multiples of eight, with a minimum requirement of 1 million cells of material. Please confirm that your tissue/sample type is compatible with our protocols. Additional sequencing can be added at the usual sequencing rates.

PacBio Bulk and 10X Single Cell Isoform Sequencing Library Preparation

- Cost: Total costs depend on how many samples there are and whether or not Kinnex is used. Sequencing costs are determined separately and can be found on the sequencing page

   $450 First cDNA (Bulk-RNA) 

   $150 Each additional cDNA (Bulk-RNA)

   $800 Kinnex (MAS-Seq) Prep (Bulk-RNA and 10X Single Cell)
- Details: For bulk isoform sequencing, sample extraction is included. For 10X single-cell sequencing, users must submit at least 30ng of full-length cDNA prepared via 10X protocols. Sequencing costs will depend on number of SMRT cells required. Contact us for a custom estimate based on your specific needs.

Upcoming Services

Illumina Bulk RNA-seq

- Details: This service will include RNA extraction with DNase treatment, ribosomal RNA depletion library preparation, and sequencing with 30 million paired-end 150 bp (PE150) reads. 

PacBio HiFi Whole Genome Sequencing

- Details: This includes DNA extraction, DNA fragmentation, library preparation, and high-fidelity (HiFi) 30x genome sequencing, with optional 5mC methylation calls for epigenetic analysis.

For any questions or custom requirements, feel free to reach out to our team for more information. We’re here to help you get the most from our sequencing services! 

* Rates are higher for non-UC academic/non-profit groups (+26%) and for-profit groups (+57.5%). Bioinformatic analysis services can also be added

Referencing the CAT Core and Publications

 

When submitting manuscripts, please acknowledge the CAT by including the text: “Sequencing was performed at the UCSF CAT, supported by UCSF PBBR, RRP IMIA, and NIH 1S10OD028511-01 grants.” 

 

To aid our facility in future grant submissions, it helps to reference labs and publications that have benefited from our core please notify us of your achievement by emailing catcore [at] ucsf.edu the PMID number, lab, and the piece of equipment used in the paper.