Introduction to Firefly Assay Development Wiki
The Firefly is an automated 
isoelectric focusing instrument made by Cell Biosciences.
Grouped by topic. Feel free to add one!
This page was updated by Liz on 27 Jul 07
There is an IBM laptop on the lab bench near the Firefly which contains several useful Firefly protocols and information about running a Firefly assay and managing Firefly data. These files are located on the Desktop.
You are welcome to contact Liz for more information about using Firefly at the CAT:
Liz Gaudet, Ph.D.
Application Scientist
Cell Biosciences, Inc.
1050 Page Mill Road
Palo Alto, CA 94303-0803
lgaudet@cellbiosciences.com
click on the hyperlinks to go to the following chapters:
- FireflySamplePreparation
- FireflyInstrumentTroubleshooting?
- TrainingProtocolErk? with Control LNCaP lysate and Erk Abs
Protocols - All available at http://cat.ucsf.edu/equipment/firefly/index.html
Firefly Assay Cell Lysis and Quantitation Protocol
Firefly Assay Flowchart and Representative Images
Probing for MEK and ERK
Lambda Phosphatase Reaction Setup
How to Send Firefly Data with YouSendIt
How to Use ChemiView to View Firefly Tifs
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