Core Facility > Resources
Community Oligo Sets
Several oligo sets are available in our facility for the UCSF community to use. The Operon S. cerevisiae AROS and YBOX oligo sets, the Invitrogen Mouse Exonic Evidence Based Oligo (MEEBO) set, and the Invitrogen Human Exonic Evidence Based Oligo (HEEBO) set. UCSF users have free access to these oligo sets. Non-UCSF academic users are required to pay a fee to use the sets. Currently, the non-UCSF academic price for using the S. cerevisiae AROS/YBOX set is $1000 for a 261-slide printrun or $500 for a 130-slide printrun. In addition, all users are required to replace any plates damaged during the course of their work. Please contact Clement Chu if you would like access to one of these oligo sets.
- AROS Oligo Set (in order)
- YBOX Oligo Set (in order)
- MEEBO Oligo Set (in order)
- HEEBO Oligo Set (in order)
- Stanford S. cerevisiae Godlist - S. cerevisiae oligo IDs and annotations used by Stanford University. While the oligo sets are the Operon AROS and YBOX oligo sets, the Stanford group has used a slightly different nomenclature for naming the genes. If you have been using arrays printed by Stanford (Oligo Print 1 and Print 2), then this list may be helpful when you prepare to publish your data.
- UCSF S. cerevisiae Godlist - Combination of AROS and YBOX oligos being printed at UCSF. Document incorporates all of the information in the Operon AROS and YBOX annotations as of 8/31/2007. It also connects the Stanford unique IDs to the Operon unique IDs.
Community Microarrays
S. cerevisiae microarrays are often available in the CAT for UCSF community members. Each lab is allowed 20 arrays at no charge. Once the lab exceeds 20 arrays, then the lab's name goes on a list of potential volunteers to help with the next printrun. When the current printrun runs low, the lab will be contacted about helping with the next printrun. If you would like more information, please contact Clement.
Operon AROS Oligo Arrays
Printed 11/2006 by UCSF Biophysics Class
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- GPS File
Operon AROS + YBOX Arrays
yOligo-P02 Printed 2/2007
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- Gal File
yOligo-P01 Printed 4/2006
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- Gal File
Yeast ORF Arrays
yORF-p06 Printed 1/2006
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- Gal File
- To save any of the above files, right-click and "Save Link As"
Software
useful programs, free downloads
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- Cluster
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- heirarchical clustering and SOMs for microarray data
- now available for more platforms (PC, Linux, Mac OS X)
- Treeview
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- visualization of clustered for microarray data
- Java powered -- will run on all platforms
- NOMAD
- MySQL database for microarray data (linux) (UCSF)
- LAD
- postgreSQL database for microarray data (linux) (UT Austin)
- ArrayMaker v2.60 - for operating a microarrayer (PC)Download
- GalFileGenerator version 1.4
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- produces Genepix array list (.gal) from microarray print list (PC)
- can now space rows correctly for orange-packed arrays
Links
- UCSF Core Facilities
- Research Core Facilities at UCSF
- - a comprehensive list of UCSF core facilities
- UCSF Sandler Center for Functional Genomics Core Facility
- - a UCSF core facility that assists users with particular grant sources with microarray studies
- Nikon Imaging Center
- - a state-of-the-art imaging center housed in the CAT
- Lab for Cell Analysis
- - UCSF Core Facility for cytometric analysis
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- Other Useful Links
- UCSF Bioinformatics Services
- - provides services to help UCSF investigators plan and analyze datasets generated from high-throughput techniques
- UCSF Center for Bioinformatics & Molecular Biostatistics:
- Statistical Methods in Bioinformatics Course, Fall 2006
- Gladstone Genomics Core Laboratory
- - specialize in Affymetrix Array Platform
- OpenWetWare.org
- - Wikipedia-like site for biologists; a great resource for protocols and information
- NOMAD 2
- - UCSF NOMAD 2 database for UCSF users
- Stanford Genomic Resources
Protocols
- Microarrayer
- the M-guide: building a microarrayer v3.1
- a brief Maintenance Manual for the Microarrayer v1.0
- drivers for Galil DMC-18x2, circa 1999 (required for ArrayMaker)
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- Printing Microarrays
- Print Manual: Printing with the Microarrayer v2.3
- Preparing Poly-lysine Slides
- S. cerevisiae genome PCR amplification
- Precipitating PCR-amplified DNA for arraying
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- Hybridizing Microarrays
- Microarray Overview v2.7
- DNA resuspension for microarraying
- Using the MAUI for Hybridization
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- Amino allyl labeling of genomic DNA 1
- Amino allyl labeling of genomic DNA 2
- RNA amplification
- DNA amplification
- MEEBO: aRNA amplification and hybridization
- Array Scanning with GenePix
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- Tips for S. cerevisiae experiments
- Growing S. cerevisiae for microarray experiments
- Isolating S. cerevisiae total RNA
- Isolating S. cerevisiae poly-A RNA
- Immunoprecipitation of S. cerevisiae chromatin
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- Isolating human RNA
- Labeling human RNA
- Isolating human tumor total RNA
- Isolating membrane-bound polysomes from tissue culture cells
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- Amplifying the S. cerevisiae Genome
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- Malaria Protocols
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