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Core Facility > Resources

Community Oligo Sets

Several oligo sets are available in our facility for the UCSF community to use. The Operon S. cerevisiae AROS and YBOX oligo sets, the Invitrogen Mouse Exonic Evidence Based Oligo (MEEBO) set, and the Invitrogen Human Exonic Evidence Based Oligo (HEEBO) set. UCSF users have free access to these oligo sets. Non-UCSF academic users are required to pay a fee to use the sets. Currently, the non-UCSF academic price for using the S. cerevisiae AROS/YBOX set is $1000 for a 261-slide printrun or $500 for a 130-slide printrun. In addition, all users are required to replace any plates damaged during the course of their work. Please contact Jennifer Shock if you would like access to one of these oligo sets.
  • AROS Oligo Set (in order)
  • YBOX Oligo Set (in order)
  • MEEBO Oligo Set (in order)
  • HEEBO Oligo Set (in order)
  • Stanford S. cerevisiae Godlist - S. cerevisiae oligo IDs and annotations used by Stanford University. While the oligo sets are the Operon AROS and YBOX oligo sets, the Stanford group has used a slightly different nomenclature for naming the genes. If you have been using arrays printed by Stanford (Oligo Print 1 and Print 2), then this list may be helpful when you prepare to publish your data.
  • UCSF S. cerevisiae Godlist - Combination of AROS and YBOX oligos being printed at UCSF. Document incorporates all of the information in the Operon AROS and YBOX annotations as of 8/31/2007. It also connects the Stanford unique IDs to the Operon unique IDs.

Community Microarrays

S. cerevisiae microarrays are often available in the CAT for UCSF community members. Each lab is allowed 20 arrays at no charge. Once the lab exceeds 20 arrays, then the lab's name goes on a list of potential volunteers to help with the next printrun. When the current printrun runs low, the lab will be contacted about helping with the next printrun. If you would like more information, please contact Jennifer Shock.
Operon AROS Oligo Arrays
Printed 11/2006 by UCSF Biophysics Class
GPS File

Operon AROS + YBOX Arrays

yOligo-P02 Printed 2/2007
Gal File
yOligo-P01 Printed 4/2006
Gal File
Yeast ORF Arrays
yORF-p06 Printed 1/2006
Gal File
To save any of the above files, right-click and "Save Link As"

Software

useful programs, free downloads

Cluster
heirarchical clustering and SOMs for microarray data
now available for more platforms (PC, Linux, Mac OS X)

Treeview
visualization of clustered for microarray data
Java powered -- will run on all platforms

NOMAD
MySQL database for microarray data (linux) (UCSF)

LAD
postgreSQL database for microarray data (linux) (UT Austin)

ArrayMaker v2.60 - for operating a microarrayer (PC)Download

GalFileGenerator version 1.4
produces Genepix array list (.gal) from microarray print list (PC)
can now space rows correctly for orange-packed arrays

Links

UCSF Core Facilities
Research Core Facilities at UCSF
- a comprehensive list of UCSF core facilities
UCSF Sandler Center for Functional Genomics Core Facility
- a UCSF core facility that assists users with particular grant sources with microarray studies
Nikon Imaging Center
- a state-of-the-art imaging center housed in the CAT
Lab for Cell Analysis
- UCSF Core Facility for cytometric analysis
 
Other Useful Links
UCSF Bioinformatics Services
- provides services to help UCSF investigators plan and analyze datasets generated from high-throughput techniques
UCSF Center for Bioinformatics & Molecular Biostatistics:
Statistical Methods in Bioinformatics Course, Fall 2006
Gladstone Genomics Core Laboratory
- specialize in Affymetrix Array Platform
OpenWetWare.org
- Wikipedia-like site for biologists; a great resource for protocols and information
NOMAD 2
- UCSF NOMAD 2 database for UCSF users
Stanford Genomic Resources

Protocols

Microarrayer
the M-guide: building a microarrayer v3.1
a brief Maintenance Manual for the Microarrayer v1.0
drivers for Galil DMC-18x2, circa 1999 (required for ArrayMaker)
 
Printing Microarrays
Print Manual: Printing with the Microarrayer v2.3
Preparing Poly-lysine Slides
S. cerevisiae genome PCR amplification
Precipitating PCR-amplified DNA for arraying
 
Hybridizing Microarrays
Microarray Overview v2.7
DNA resuspension for microarraying
Using the MAUI for Hybridization
 
Amino allyl labeling of genomic DNA 1
Amino allyl labeling of genomic DNA 2
RNA amplification
DNA amplification
MEEBO: aRNA amplification and hybridization
Array Scanning with GenePix
 
Tips for S. cerevisiae experiments
Growing S. cerevisiae for microarray experiments
Isolating S. cerevisiae total RNA
Isolating S. cerevisiae poly-A RNA
Immunoprecipitation of S. cerevisiae chromatin
 
Isolating human RNA
Labeling human RNA
Isolating human tumor total RNA
Isolating membrane-bound polysomes from tissue culture cells
 
Amplifying the S. cerevisiae Genome
 
Malaria Protocols
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