The Biacore T100 is one of two surface plasmon resonance instruments available through the CAT for real-time monitoring of protein-protein and protein-small molecule interactions. These instruments enable the measurements of binding affinity (Kd values) and binding kinetics (kon and koff), concentration determinations, binding specificity analyses, and epitope mapping. In comparison to other assay techniques for binding interactions, SPR requires smaller amounts of sample and is rapid, sensitive, versatile, and label-free. With many improvements in biosensor technology and experimental practices, SPR has become widely accepted for generating reliable and publishable kinetic data on interactions between biological molecules. These data are highly valued in defining discrete structural components of complex interactions.
How does the Biacore T100 differ from the other SPR instruments available in the facility? The Biacore T100 has increased sensitivity, temperature controls, and automation than the 1000 instrument. However, the sensor chips are more expensive than those for the 1000 model, and it is more likely to be booked.
You must be trained before you schedule or begin using the Biacore T100. The Biacore instruments are available for investigator-operated and investigator-assisted use. Prior to investigator-operated use, the operator must have completed a Biacore training course or have demonstrated expertise (publications) in the field. Specific training on the use of our instruments is provided after users have taken a Biacore course or demonstrated prior experience with Biacore instruments. For more information on whether this assay is appropriate for your question, interested users are encouraged to check out training CD-ROMs and pertinent literature from the CAT facility.
You will need your own Biacore sensor chips. We do not provide any disposables for general use.
Location - GH-S401A
- Charge
- The charges for this instrument are still being determined. For up-to-date information, please contact Peter Hwang.
